Middleware groups in Laravel let you run many middleware one after another on a request. When a request comes in, Laravel checks which middleware group applies. It runs each middleware in that group step by step. For example, the 'web' group runs middleware like EncryptCookies, StartSession, and VerifyCsrfToken before the controller handles the request. If all middleware pass, the controller runs and returns a response. If any middleware fails, the request stops and the controller does not run. This helps keep requests safe and prepared. You define middleware groups in your route files using Route::middleware(['group'])->group(...).