The PRIMARY KEY constraint is used in SQL to uniquely identify each row in a table. When creating a table, you specify one or more columns as the PRIMARY KEY. This constraint ensures that no two rows can have the same value in the PRIMARY KEY column(s), and that these values cannot be NULL. During data insertion, the database checks if the PRIMARY KEY value already exists or is NULL. If so, it rejects the insertion with an error. This maintains data integrity by preventing duplicate or missing identifiers. For example, in the Students table, the ID column is the PRIMARY KEY. Inserting a row with ID=1 succeeds the first time but fails if attempted again or if NULL is used. This behavior is critical for reliable database operations.