In Laravel, you define routes in the routes/web.php file. When a user visits a URL, Laravel reads this file to find a matching route. If it finds one, it runs the code inside the route's closure and sends the result back to the browser. If no route matches, Laravel returns a 404 Not Found error. For example, defining Route::get('/', function () { return 'Hello, world!'; }); means when the home URL '/' is requested, Laravel sends 'Hello, world!' as the response. If the user requests a URL like '/about' without a matching route, Laravel returns 404. Adding a route for '/about' changes this behavior to return the new response instead.