In Express, sanitization methods help clean user input to prevent harmful data from causing problems. First, input is trimmed to remove extra spaces. Then, escape converts characters like < and > into safe codes so scripts can't run. Validation checks if input is okay. If all is good, the sanitized input is used safely in the app. This process protects your app from attacks like cross-site scripting. The example code shows trimming and escaping a username before sending it back in a response. The execution table traces each step, showing how the input changes and when it is safe. Remember, always sanitize and validate input to keep your app secure.