In Django, middleware are called in the order they are listed for processing requests. Each middleware's process_request method runs one after another from top to bottom. After all middleware process the request, the view handles it and returns a response. Then, the response goes back through middleware in reverse order, calling each middleware's process_response method from bottom to top. This ordering means the first middleware listed processes the request first but processes the response last. Changing the order of middleware changes how the request and response are modified. If a middleware returns a response early during process_request, the view and later middleware process_request methods are skipped, and process_response methods run in reverse order up to that middleware. Understanding this flow helps avoid bugs and ensures middleware behave as expected.