The @Size annotation in Spring Boot is used to check if a string field's length is within specified minimum and maximum limits. When an object is created or validated, the field value's length is measured. If the length is less than the minimum or greater than the maximum, validation fails and an error is returned. Otherwise, validation passes. For example, a username with @Size(min=3, max=10) must have length between 3 and 10 characters. If the username is "Jo" with length 2, validation fails because it is too short. If the username is "John" with length 4, validation passes. This helps ensure that user input meets length requirements before processing.