In Flask, routes are defined by decorating functions with @app.route and specifying a URL string. The URL should be lowercase and use slashes to separate parts, like '/user/profile'. The function name should use snake_case, like user_profile, following Python style. Flask matches URLs exactly, so '/User/Profile' will not match '/user/profile' and cause a 404 error. This example shows defining a route, registering it, and handling a request. The execution table traces these steps and highlights the importance of naming conventions for URLs and functions to avoid errors and improve clarity.